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. 2020 Aug 14;18(8):e3000814. doi: 10.1371/journal.pbio.3000814

Fig 3. Response to purified cCF10 is sensitive and costly.

Fig 3

(A) A phenotypic assay for Asc10 activity (see Adherence/growth assay) showing the macroscopic appearance of static cultures of OG1RF donors carrying wild-type pCF10 or the pCF10-GFP Asc10 expression reporter [23] after an overnight incubation with varying concentrations of purified cCF10. Unstimulated cells appear as a precipitate in the center of the microwell. Adhered cells appear as a film. (B) Time-resolved microwell horizontal spatial scanning (11 positions) of optical density in OG1RF(pCF10). The underlying numerical data are shown in S4 Data. GFP, green fluorescent protein.