Table 1:

Suggested dilutions series for a VSV stock with an estimated titer of 10⁹ PFU/ml.

Dilution #	μl virus from previous dilution	μl media	Total dilution	Expected # plaques
1	10 μl of virus stk	990	1X10−2
2	10 μl from tube 1	990	1X10−4
3	50 μl from tube 2	450	1X10−5	1000
4	50 μl from tube 3	450	1X10−6	100
5	250 μl from tube 4	250	5X10−8	50
6	250 μl from tube 5	250	2.5X10−8	25
7	250 μl from tube 6	250	1.25X10−8	13