FIG 3.

In situ visualization of Histoplasma reveals unique intracellular growth dynamics. (A to C) Lungs were harvested on day 8 (A), day 12 (B), or day 16 (C) after inoculation with either gfp-expressing H. capsulatum or H. ohiense. Immunohistochemistry was performed on serial sections to chromogenically detect anti-GFP followed by hematoxylin staining. Red circles in panel A highlight heavily infected mononuclear phagocytes in the lungs of H. ohiense-infected mice, and arrowheads point to cells associated with few yeasts per cell. Blue and red arrowheads in panel C highlight yeasts detected within lung inflammatory foci 16 days after inoculation. p.i., postinoculation.