TABLE 1.
Strains and plasmids used in this study
| Strain, plasmid, or element |
Relevant genotype or phenotypea | Reference |
|---|---|---|
| Vibrio cholerae | ||
| HC-36A1 | Clinical, non-O1/non-O139, Haiti, 2010 (Ap Cm Kn Sp Sm Su Tc Tm) | 57 |
| Escherichia coli | ||
| BW25113 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514 | 75 |
| GG56 | Nxr derivative of BW25113 (Nx) | 76 |
| VB112 | Rfr derivative of MG1655 (Rf) | 40 |
| CAG18439 | MG1655 lacZU118 lacI42::Tn10 (Tc) | 77 |
| VB47 | CAG18439 ΔrecA ΔgalK | 78 |
| JW3642 | BW25113 ΔuhpC772::aph (Kn) | 18 |
| JW3692 | BW25113 ΔpurP745::aph (Kn) | 18 |
| JW3718 | BW25113 ΔgidB769::aph (Kn) | 18 |
| JW5858 | BW25113 ΔyidX732::aph (Kn) | 18 |
| Plasmids | ||
| pVCR94 | IncC conjugative plasmid, V. cholerae O1 El Tor (Su Tm Cm Ap Tc Sm) | 10 |
| pVCR94Sp | Spr derivative of pVCR94 (pVCR94ΔX2) (Su Sp) | 9 |
| pVCR94Kn | Knr derivative of pVCR94 (pVCR94ΔX3) (Su Kn) | 9 |
| pVCR94Cm | Cmr derivative of pVCR94 (pVCR94ΔX4) (Su Cm) | 17 |
| pVCR94ΔmobIC | mobIC deletion mutant of pVCR94Sp (Su Sp) | This study |
| pVCR94ΔtraI | traI deletion mutant of pVCR94Sp (Su Sp) | This study |
| pVCR94ΔtraD | traD deletion mutant of pVCR94Sp (Su Sp) | This study |
| pVCR94ΔtraJ | traJ deletion mutant of pVCR94Sp (Su Sp) | This study |
| pVCR94ΔtraN | traN deletion mutant of pVCR94Sp (Su Sp) | 9 |
| pVCR94ΔtraG | traG deletion mutant of pVCR94Sp (Su Sp) | 9 |
| pVCR94ΔtraH | traH deletion mutant of pVCR94Sp (Su Sp) | 9 |
| pBAD30 | orip15A bla araC PBAD (Ap) | 79 |
| pacaCD | pBAD30::acaDC (Ap) | 9 |
| pBAD-mobIC | pBAD30::mobIC (Ap) | This study |
| pBAD-traD | pBAD30::traD (Ap) | This study |
| pBAD-traJ | pBAD30::traJ (Ap) | This study |
| pBAD-traN | pBAD30::traN (Ap) | 17 |
| pBAD-traG | pBAD30::traG (Ap) | 17 |
| pBAD-traH | pBAD30::traH (Ap) | 17 |
| pBAD-int | pBAD30::int (Ap) | This study |
| pBAD-xis | pBAD30::xis (Ap) | This study |
| pT84 | pBAD-TOPO::84 (Ap) | This study |
| pBAD-84 | pBAD30::84 (Ap) | This study |
| pBAD-84-31 | pBAD30::84 starting at position +31 of 84 (Ap) | This study |
| pBAD-84-106 | pBAD30::84 starting at position +106 of 84 (Ap) | This study |
| pBAD-84-118 | pBAD30::84 starting at position +118 of 84 (Ap) | This study |
| pBAD-84-304 | pBAD30::84 starting at position +304 of 84 (Ap) | This study |
| pBAD-84-409 | pBAD30::84 starting at position +409 of 84 (Ap) | This study |
| pOPlacZ | oriR6Kγ attPλ aph lacZ (Kn) | 9 |
| pPromint | pOPlacZ containing −163 to −12 of MGIVchHai6 Pint (Kn) | This study |
| pProm85 | pOPlacZ containing −389 to −11 of MGIVchHai6 P85 (Kn) | This study |
| pPrommobI | pOPlacZ containing −345 to −11 of MGIVchHai6 P84 (Kn) | This study |
| pPromtraHS | pOPlacZ containing −243 to −14 of SGI1 PtraHs (Kn) | 17 |
| pDC1 | pACYC184 Δcat (ΔMscI-PvuII) (Tc) | 40 |
| pIG0-84 | pDC1::IG0-84 (Tc) | This study |
| pIG0-84G120C | pDC1::IG0-84 G120C (Tc) | This study |
| pIG0-84G306C | pDC1::IG0-84 G306C (Tc) | This study |
| pIG0 | pDC1::IG0 (Tc) | This study |
| pIG1 | pDC1::IG1 (Tc) | This study |
| pIG2 | pDC1::IG2 (Tc) | This study |
| pIG3 | pDC1::IG3 (Tc) | This study |
| pIG4 | pDC1::IG4 (Tc) | This study |
| pIG5 | pDC1::IG5 (Tc) | This study |
| pIG6 | pDC1::IG6 (Tc) | This study |
| pIG7 | pDC1::IG7 (Tc) | This study |
| pIG8 | pDC1::IG8 (Tc) | This study |
| pIG9 | pDC1::IG9 (Tc) | This study |
| pIG10 | pDC1::IG10 (Tc) | This study |
| pIG11 | pDC1::IG11 (Tc) | This study |
| pIG12 | pDC1::IG12 (Tc) | This study |
| pIG13 | pDC1::IG13 (Tc) | This study |
| pINT-ts | oriR101 cI857 λpR-intλ (ts, Ap) | 80 |
| pSIM6 | λRed recombination thermo-inducible encoding plasmid (ts, Ap) | 81 |
| pMS1 | λRed recombination thermo-inducible encoding plasmid (ts, Gm) | 10 |
| pKD3 | cat (Cm) template for one-step chromosomal gene inactivation | 75 |
| pKD4 | aph (Kn) template for one-step chromosomal gene inactivation | 75 |
| pCP20 | Flp recombinase thermo-inducible encoding plasmid (ts, Ap Cm) | 82 |
| pCP20-Gm | Gmr derivative of pCP20 (ts, Gm Cm) | 83 |
| Genomic islands | ||
| MGIVchHai6 | Drug resistance island, V. cholerae HC-36A1 (Ap Cm Sm Su Tm Tc) | 13 |
| MGIKn | Knr derivative of MGIVchHai6 lacking In36A1 and Tn6310 (Kn) | This study |
| MGICm | Cmr derivative of MGIVchHai6 lacking In36A1 and Tn6310 (Cm) | This study |
| MGIKnΔint | int deletion mutant of MGIKn (Kn) | This study |
| MGIKnΔxis | xis deletion mutant of MGIKn (Kn) | This study |
| MGICmΔxis | xis deletion mutant of MGICm (Cm) | This study |
| MGIKnΔ86 | 86 deletion mutant of MGIKn (Kn) | This study |
| MGIKnΔ85 | 85 deletion mutant of MGIKn (Kn) | This study |
| MGIKnΔ84 | 84 deletion mutant of MGIKn (Kn) | This study |
a
Ap, ampicillin; Cm, chloramphenicol; Gm, gentamicin, Kn, kanamycin; Nx, nalidixic acid; Rf, rifampin; Sp, spectinomycin; Sm, streptomycin; Su, sulfamethoxazole; Tc, tetracycline; Tm, trimethoprim; ts, thermosensitive.