TABLE 2.
DNA sequences of the primers used in this study
| Primer name | Nucleotide sequence (5′ to 3′)a | Reference |
|---|---|---|
| 94delmobI.for | ATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGGTGTAGGCTGGAGCTGCTTC | This study |
| 94delmobI.rev | TCCCCAGTTTCGCCAATTCAGTGGCCGCTACAGATGCTGTCATATGAATATCCTCCTTA | This study |
| 94deltraI.for | TGTAGCGACGAGAAATACGATAGGAATATGGTCAACCTACGTGTAGGCTGGAGCTGCTTC | This study |
| 94deltraI.rev | CACGGCATCTCGTAGGCGAGCGGGTCATAACTCATTGTCATATGAATATCCTCCTTA | This study |
| 94deltraD.for | CAGGGAAAATTGTATTTAGAGGTAAATGAAACATGAGTGTAGGCTGGAGCTGCTTC | This study |
| 94deltraD.rev | TCTCTATACTTCTTGTCACACATAGCTCTGCTCTTACATATGAATATCCTCCTTA | This study |
| 94deltraJ.for | GCCGTGGTTGCTGGTCGCATGGCTGCTGGTTATTGAGTGTAGGCTGGAGCTGCTTC | This study |
| 94deltraJ.rev | ATTTATAACGGAGTAGCTCATCCTCCCTCCCTATACCATATGAATATCCTCCTTA | This study |
| Hai6delWE.for | TGCCGTAATCAAGGAAATGATACGGCAGAAAGTCGTGGAACGGAATAGGAACTTCAAGAT | This study |
| Hai6delWE.rev | TGATTACGACTACCGAAACCATATTGGGTTTAATGACTGAAGGAACTTCAGAGCGCTT | This study |
| Hai6delint.for | CTGGCGCACATATGGCGCACAAGATGAGAAAAACCTGTGTAGGCTGGAGCTGCTTC | This study |
| Hai6delint.rev | TTGTAAAGATGTTGTCGTTTGATCGTAATAATGCTCTTACATATGAATATCCTCCTTA | This study |
| Hai6delxis.for | TCCCACTTTAAGTTGGCAGTGTAGGAGAGTATCAACGTGTAGGCTGGAGCTGCTTC | This study |
| Hai6delxis.rev | CCATTCATATTTATTTTCAAAAGGTTAAATCTCAACTTACATATGAATATCCTCCTTA | This study |
| Hai6del86.for | GCTGCATGGTTGAGTGATTTATAAAAGGGGGAAGTGGTGTAGGCTGGAGCTGCTTC | This study |
| Hai6del86.rev | TTAAAGTGGGATAAACGGGAAACTGGGAACACTAAGTTACATATGAATATCCTCCTTA | This study |
| Hai6del85.for | TTACTCCTGCAATCTAGATAATAAACAGGAGCCATCGTGTAGGCTGGAGCTGCTTC | This study |
| Hai6del85.rev | CTGCAACTTTTTTTGCATCACTTCCCCCTTTTATAACATATGAATATCCTCCTTA | This study |
| Hai6delmobI.for | ATCAGATAGTTATTGGAAAGGAATTGGTAGGGTCTTGTGTAGGCTGGAGCTGCTTC | This study |
| Hai6delmobI.rev | CTCTAATGAGTTGAGGGCTGTGTTGTAAGATCATAATTACATATGAATATCCTCCTTA | This study |
| 94mobIEcoRI.for | NNNNNN GAATTC AAGGAGGAATAATAAATGAGTCTACCAACAGAGCGATGGCT | This study |
| 94mobIEcoRI.rev | NNNNNN GAATTC TCACACCTCGTCGCTATGTGTCTT | This study |
| 94traD61EcoRI.for | NNNN GAATTC AAGGAGGAATAATAAATGACAATGAGTTATGAC | This study |
| 94traD61EcoRI.rev | NNNN GAATTC TTACTGGGTCAATATGGGCAGAC | This study |
| 94traJ63EcoRI.for | NNNN GAATTC AAGGAGGAATAATAAATGAAGAAGCCGTGGTTGCTG | This study |
| 94traJ63EcoRI.rev | NNNN GAATTC CTATACCCGTTTCTGGAGGTTG | This study |
| Hai6intEcoRI.for | NNNN GAATTC AAGGAGGAATAATAAATGAAAATCAGCATACATAAAC | This study |
| Hai6intEcoRI.rev | NNNN GAATTC TTAGTCTGATGACTCATCGAAG | This study |
| Hai6xisEcoRI.for | NNNN GAATTC AAGGAGGAATAATAAATGACTGAAAAAGAGATGCTTC | This study |
| Hai6xisEcoRI.rev | NNNN GAATTC CTAATATCTCCGGCCATAC | This study |
| Hai6mobIXbaI.for | NNNNNN TCTAGA AAGGAGGAATAATAAATGGCTAAGGCGTCACAACAAAA | This study |
| Hai6mobIXbaI.rev | NNNNNN TCTAGA TTATAAAGGATTATCCATCTTAAAATCGTCGAT | This study |
| Hai6mobINcoI.for | CCATGG CTAAGGCGTCACAAC | This study |
| Hai6mobI.rev | TTATAAAGGATTATCCATCTTAAAATCGTCGAT | This study |
| Hai6promintPstI.f | NNNNNN CTGCAG AATAGTAAAACACATCAAACC | This study |
| Hai6promintPstI.r | NNNNNN CTGCAG ATCTTGTGCGCCATATGTGCG | This study |
| Hai6prom85PstI.f | NNNNNN CTGCAG AATAACTATCTGATGGGGGAT | This study |
| Hai6prom85PstI.r | NNNNNN CTGCAG GTTTATTATCTAGATTGCAGG | This study |
| Hai6prom84PstI.f | NNNNNN CTGCAG AATGAGTTGCTCGACATTAC | This study |
| Hai6prom84PstI.r | NNNNNN CTGCAG ACTGTATGTTGAATACAGAG | This study |
| 43_attPF | GCAATTAATGATAAAGACGGGTA | 13 |
| Ec104D.rev | AACCATTTTGAGGTCACACA | 15 |
| Hai6_attPR | TCAAATCACTTCCCACCAAG | This study |
| Start_mobI_F1 | GTGCCCCAAAAGGGCACGAAG | This study |
| Start_mobI_F2 | GTGGATTATGTCATGACCAA | This study |
| Start_mobI_F3 | ATGACCAAAAATTATTCATTTTTAG | This study |
| Start_mobI_F4 | ATGCTTTTTTCATTATCAGTTTCAG | This study |
| Start_mobI_F5 | TTGTTCTGGCAGCACAACCGA | This study |
| Start_mobI_Rv2 | TTATTATTCCTCCTTTCTAGAGGATC | This study |
| Hai6oriT84XbaI.for | NNNNNN TCTAGA TTGCAGGAGTAATCATCTCGAAAAA | This study |
| mobI120_GC.F | ATTATGTCATCACCAAAAATTATTCATTTTTAG | This study |
| mobI120_GC.R | CCACTGTATGTTGAATACAG | This study |
| mobI306_GC.F | ACGATGCTATCCTTTTTTCATTATC | This study |
| mobI306_GC.R | CTTGATTAACGTTTGTGC | This study |
| Hai6oriTXbaI.rev | NNNNNN TCTAGA AAGACCCTACCAATTCCTTTC | This study |
| minoriT1.F | CACGCTGATATTTAATGATATTTATTC | This study |
| minoriT1-4a.R | TCTAGAAATATTTTATCTGATTAATAAGATG | This study |
| minoriT2.F | AGCCACCAATGAGTTGCT | This study |
| minoriT3.F | CCGTATCAATTTATGCGC | This study |
| minoriT4b.F | TCTAGATTTCAGTGCAATTTATC | This study |
| minoriT4b.R | ACGATTTATTCCATACAAGC | This study |
| minoriT5.F | CTCAGGTCTTTTATTGGC | This study |
| minoriT5.R | CTCTGGTTATTCACTGGC | This study |
| minoriT7.F | AATAAATCGTCCAAGATCC | This study |
| minoriT8.F | TCCAAGATCCCCCATCAG | This study |
| minoriT8XbaI.F | NNNN TCTAGA TCCAAGATCCCCCATCAG | This study |
| minoriT9XbaI.R | NNNN TCTAGA GACATAATCCACTGTATGTTG | This study |
| minoriT11.R | ACGGGTCTTTTGTTGTGAC | This study |
| Hai6delWEcm.for | TGCCGTAATCAAGGAAATGATACGGCAGAAAGTCGTGGAATACCTGTGACGGAAGATCAC | This study |
| Hai6delWEcm.rev | TGATTACGACTACCGAAACCATATTGGGTTTAATGACTGATAGGAACTTCATTTAAATGG | This study |
a
Restriction sites are underlined.