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. 2020 Aug 26;6(35):eaaz5752. doi: 10.1126/sciadv.aaz5752

Fig. 5. SQR mediates therapeutic effects of H2S by targeting mitochondrial electron transfer at complex I to activate AMPK in vitro.

Fig. 5

(A to C) Protein (n = 4) or mRNA (n = 3) levels of proinflammatory mediators in BV2 microglia transfected with SQR-siRNA (small interfering RNA) or nonsense siRNA (Ctrl-siRNA) for 2 days and then treated with the RBC lysate (LY) and ADT-OH/Veh. (D) SQR knockdown efficiency in BV2 microglia. (E and F) Immunoblot images of AMPK activation in BV2 microglia transfected with SQR-siRNA or Ctrl-siRNA and then treated with LY and ADT-OH/Veh (E) and quantification data (F). (G to I) Protein or mRNA levels of proinflammatory mediators in BV2 microglia transfected with AMPK-siRNA or Ctrl-siRNA and then treated with LY and ADT-OH/Veh; n = 4. (J) AMPK knockdown efficiency in BV2 microglia. (K to M) Protein levels of proinflammatory mediators in BV2 microglia transfected with NDUFS3-siRNA or Ctrl-siRNA and then treated with LY and ADT-OH/Veh; n = 4. (N and O) Immunoblot images of AMPK activation in BV2 microglia transfected with NDUFS3-siRNA or Ctrl-siRNA and then treated with LY and ADT-OH/Veh (N) and quantification data (O). (P) NDUFS3 knockdown efficiency in BV2 microglia.