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. 2020 Aug 26;6(35):eaba7637. doi: 10.1126/sciadv.aba7637

Fig. 2. Crystal structure of YeeE.

Fig. 2

(A and B) Overall structure of StYeeE in cartoon (A) and ribbon (B) representations from the membrane side. The transparency of H1, H3, H8, and H10 in (A) is 30% to clearly display the inside. Numbers of α helices are indicated, and YeeE characteristic loops (LA–LD) are highlighted in red and magenta. Thiosulfate ions are shown as a space-filling model. (C) YeeE structure in ribbon representation from the outside. (D) Close-up view of thiosulfate-binding site. 2FoFc map and thiosulfate-omit (FoFc) map are shown with 1.2 σ and 4.0 σ, respectively. (E) Surface model of YeeE viewed from the outside, colored to indicate electrostatic potential ranging from blue (+10 kT/e) to red (−10 kT/e). (F) Schematic topology model of YeeE. YeeE has four short α helices (H2, H5, H9, and H12), the LA–LD loop, and nine transmembrane α helices (H1, H3, H4, H6, H7, H8, H10, H11, and H13).