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. 2020 Aug 26;6(35):eaaz4551. doi: 10.1126/sciadv.aaz4551
Mettl6 gRNA FWD CACCGCTACAATGGCTTCTTTCCAA
Mettl6 gRNA REV aaacTTGGAAAGAAGCCATTGTAGC
Mettl6_scr_FWD GCAGGCAGGTTGTTAGAGAATCACT
Mettl6_scr_REV CAAATCAGAACTCTCCATAGGTACA
Mettl6_InsertScr_FWD tgatgggcatggacgagctg
Mettl6_InsertScr_REV aagtccacaccgttgatggagc
mNeonGreen-Poly donor with homology arms CCTAAGTAGCATCGTATCCTGTTGGAGCTGGAAAGTCACCTGGGGAAACTGCATCAGAGAAACTCCAGTGTCTACAATCTCAGCATCGTTTGTTCAGATGGTTGAAGAACAGAGGACCTATAGCAGCATTGAATGTGTTTCCGGCAACTACAATGGCTTCTTTCgtgagcaagggcgaggaggataacatggcctctctcccagcgacacatgagttacacatctttggctccatcaacggtgtggactttgacatggtgggtcagggcaccggcaatccaaatgatggttatgaggagttaaacctgaagtccaccaagggtgactccagttctccccctggattctggtccctcatatcgggtatggcttccatcagtacctgccctaccctgacgggatgtcgcctttccaggccgccatggtagatggctccggataccaagtccatcgcacaatgcagtttgaagatggtgcctcccttactgttaactaccgctacacctacgagggaagccacatcaaaggagaggcccaggtgaaggggactggtttccctgctgacggtcctgtgatgaccaactcgctgaccgctgcggactggtgcaggtcgaagaagacttaccccaacgacaaaaccatcatcagtacctttaagtggagttacaccactggaaatggcaagcgctaccggagcactgcgcggaccacctacacctttgccaagccaatggcggctaactatctgaagaaccagccgatgtacgtgttccgtaagacggagctcaagcactccaagaccgagctcaacttcaaggagtggcaaaaggcctttaccgatgtgatgggcatggacgagctgtacaagtaagatccagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttCAAAGGAAAGGACTGCAAGCAAGGATTCTTAGCACTGAAGAAGAGGAGAAACTGAAAAGAGACCAAGCTTTGGTGTCTGCTTTTAAACAGCAAAAACTGGAAAAAGAGGCTCAGAAGAACTGGGACCTTTTTTATAAAAGAAACAGTACCAATTTTTTCAAGGACAGACACTGGACCACCAGAGAGTTTGAGGAGTTGAGATCATGTAGGGAGGTAGGCTGGGGTTTCTCCTGGCTCGAGCCATCTTCATTTATGATGCCTAGAAGAGAGGTCCTTTGTTACATGTAGAGAGTGCTC