T7 transcription/PCR forward primer	TGGCGTAGTCGGC
T7 transcription/PCR reverse primer	CGCGCGAAGCTTAATACGACTCACTATA
T7 transcription/PCR template wt	TGG CGT AGT CGG CAG GAT TCG AAC CTG CGC GGG GAA ACC CCA ATG GAT TTC AAG TCC ATC GCC TTA ACC ACT CGG CCA CGA CTA CGA CGG TAC CGG GTA CCG TTT CGT CCT CAC GGA CTC ATC AGG TAG TCG TGT CTC CCT ATA GTG AGT CGT ATT
T7 transcription/PCR template wt U47dC	TGG CGT AGT CGG CAG GAT TCG AAC CTG CGC GGG GAG ACC CCA ATG GAT TTC AAG TCC ATC GCC TTA ACC ACT CGG CCA CGA CTA CGA CGG TAC CGG GTA CCG TTT CGT CCT CAC GGA CTC ATC AGG TAG TCG TGT CTC CCT ATA GTG AGT CGT ATT
T7 transcription/PCR template wt C32G	TGG CGT AGT CGG CAG GAT TCG AAC CTG CGC GGG GAG ACC CCA ATG GAT TTC AAC TCC ATC GCC TTA ACC ACT CGG CCA CGA CTA CGA CGG TAC CGG GTA CCG TTT CGT CCT CAC GGA CTC ATC AGG TAG TCG TGT CTC CCT ATA GTG AGT CGT ATT