Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strainbackground Saccharomyces cerevisiae | SEY6210 | Robinson et al., 1988 | SEY6210 | Parental yeast strain (genotype: MATα leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 suc2-Δ9 lys2-801 GAL), obtained from the Emr lab |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 | Robinson et al., 1988 | SEY6210.1 | Parental yeast strain (genotype: MATa leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 suc2-Δ9 lys2-801 GAL), obtained from the Emr lab |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 rsp5Δ::HIS3 pRS415-6xHIS-TEV-3xFLAG-RSP5 | This paper | DTY557 | RSP5 wild type yeast strain, obtained from the Emr lab (previously unpublished) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 rsp5Δ::HIS3 pRS415-6xHIS-TEV-3xFLAG-RSP5-WW1* | This paper | DTY558 | RSP5 mutant yeast strain, obtained from the Emr lab (previously unpublished) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 rsp5Δ::HIS3 pRS415-6xHIS-TEV-3xFLAG-RSP5-WW2* | This paper | DTY559 | RSP5 mutant yeast strain, obtained from the Emr lab (previously unpublished) |
| Strain, strain background Saccharomyces cerevisiae |
SEY6210 rsp5Δ::HIS3 pRS415-6xHIS-TEV-3xFLAG-RSP5-WW3* | This paper | DTY560 | RSP5 mutant yeast strain, obtained from the Emr lab (previously unpublished) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art4Δ::HIS3 HXT3-GFP::TRP1 | This paper | ISY001 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art2Δ::HIS3 HXT3-GFP::TRP1 | This paper | ISY013 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1 D549,551,555R,E554R-GFP::HIS3 | This paper | ISY018 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 HXT3-MUP1-C AA545-574 D549,551,555R,E554R-GFP::TRP1 | This paper | ISY025 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art4Δ::HIS3 HXT3-MUP1-C AA545-574 D549,551,555R,E554R-GFP::TRP1 | This paper | ISY026 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art2Δ::HIS3 art4Δ::HIS3 HXT3-MUP1-C AA545-574 D549,551,555R,E554R-GFP::TRP1 | This paper | ISY027 | Mutant yeast strain generated by homologous recombination and mating/tetrad dissection (for PCR primer seeSupplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art2Δ::HIS3 HXT3-MUP1-C AA545-574 D549,551,555R,E554R-GFP::TRP1 | This paper | ISY028 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 HXT3-MUP1-C AA545-574-GFP::TRP1 | This paper | ISY029 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art4∆::HIS3 HXT3-MUP1-C AA545-574-GFP::TRP1 | This paper | ISY030 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art2Δ::HIS3 art4Δ::HIS3 HXT3-MUP1-C AA545-574-GFP::TRP1 | This paper | ISY031 | Mutant yeast strain generated by homologous recombination and mating/tetrad dissection (for PCR primer seeSupplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art2∆::HIS3 HXT3-MUP1-C AA545-574-GFP::TRP1 | This paper | ISY032 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 CAN1 D567,575R,E569,574R-GFP::HIS3 | This paper | ISY033 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 HXT3-MUP1-C AA545-574 K567,572R-GFP::TRP1 | This paper | ISY042 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art4Δ::HIS3 HXT3-MUP1-C AA545-574 K567,572R-GFP::TRP1 | This paper | ISY053 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art2Δ::HIS3 art4Δ::HIS3 HXT3-MUP1-C AA545-574 K567,572R-GFP::TRP1 | This paper | ISY054 | Mutant yeast strain generated by homologous recombination and mating/tetrad dissection (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art2Δ::HIS3 HXT3-MUP1-C AA545-574 K567,572R-GFP::TRP1 | This paper | ISY055 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art2Δ::HIS3 art4::HIS3 HXT3-GFP::TRP1 | This paper | ISY061 | Mutant yeast strain generated by homologous recombination and mating/tetrad dissection (for PCR primer seeSupplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 gcn1Δ::HIS3 | This paper | JZY001 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 gcn3Δ::HIS3 | This paper | JZY002 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 gcn5Δ::HIS3 | This paper | JZY003 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 gcn4Δ::HIS3 | This paper | OSY250 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 gcn2Δ::HIS3 | This paper | OSY354 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art2Δ::HIS3 MUP1-GFP::TRP1 | This paper | TSY3 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 art2Δ::HIS3 | This paper | TSY4 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art1Δ::HIS3 | This paper | VIY011 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art2Δ::TRP1 | This paper | VIY012 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 ART2-6xHIS-TEV-3xFLAG::HIS3 | This paper | VIY030 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 ART1-6xHIS-TEV-3xFLAG::HIS3 | This paper | VIY031 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 CAN1-GFP::HIS3 | This paper | VIY036 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 HXT3-GFP::TRP1 | This paper | VIY042 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1-GFP::TRP1 | This paper | VIY054 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art2Δ::TRP1 INA1-eGFP::HIS3 | This paper | OSY924 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1 S568,573A-GFP::TRP1 | This paper | VIY059 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art2Δ::TRP1 CAN1-GFP::HIS3 | This paper | VIY062 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art2Δ::TRP1 HXT2-GFP::HIS3 | This paper | VIY063 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art2Δ::TRP1 TAT2-GFP::HIS3 | This paper | VIY064 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art2Δ::TRP1 HXT1-GFP::HIS3 | This paper | VIY065 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art2Δ::TRP1 LYP1-GFP::HIS3 | This paper | VIY068 | Mutant yeast strain generated by homologous recombination (for PCR primer see supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1 K567,572R-GFP::TRP1 | This paper | VIY073 | Mutant yeast strain generated by homologous recombination (for PCR primer seeSupplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1
T552,560A-GFP::TRP1 |
This paper | VIY076 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art1Δ::HIS3 MUP1-GFP::TRP1 | This paper | VIY079 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1 K27,28R-GFP::TRP1 | This paper | VIY082 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art2Δ::TRP1 MUP1-GFP::HIS3 | This paper | VIY092 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces
cerevisiae |
SEY6210 MUP1 K27,28,567,572R-GFP::TRP1 | This paper | VIY093 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 art1Δ::HIS3 art2Δ::TRP1 | This paper | VIY107 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1-HA-TEV-GFP K1R::TRP1 | This paper | VIY200 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1 S568,573A-HA-TEV-GFP K1R::TRP1 | This paper | VIY242 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1 T552,560A-HA-TEV-GFP K1R::TRP1 | This paper | VIY255 | Mutant yeast strain generated by homologous recombination (for PCR primer see Supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1 G78N-HA-TEV-GFP K1R::TRP1 | This paper | VIY263 | Mutant yeast strain generated by homologous recombination (for PCR primer see supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210.1 ART2-6xHIS-TEV-3xFLAG::HIS3 gcn4Δ::HIS3 | This paper | VIY291 | Mutant yeast strain generated by homologous recombination and mating/tetrad dissection (for PCR primer see supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1 D43A,G47A,Q49A,T52A,L54A-HA-TEV-GFP K1R::TRP1 | This paper | VIY295 | Mutant yeast strain generated by homologous recombination (for PCR primer see supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1 T6,25,26,34A,S9,22,23,24,31,33,42A,K16,27,28R-HA-TEV-GFP K1R::TRP1 | This paper | VIY335 | Mutant yeast strain generated by homologous recombination (for PCR primer see supplementary file 4) |
| Strain, strain background Saccharomyces cerevisiae | SEY6210 MUP1-GFP::HIS3 | This paper | YSM24 | Mutant yeast strain generated by homologous recombination (for PCR primer see supplementary file 4) |
| Recombinant DNA reagent | pRS413 (Plasmid) | Sikorski and Hieter, 1989 | pRS413 | Empty centromer vector HIS3 |
| Recombinant DNA reagent | pRS414 (Plasmid) | Sikorski and Hieter, 1989 | pRS414 | Empty centromer vector TRP1 |
| Recombinant DNA reagent | pRS415 (Plasmid) | Sikorski and Hieter, 1989 | pRS415 | Empty centromer vector LEU2 |
| Recombinant DNA reagent | pRS416 (Plasmid) | Sikorski and Hieter, 1989 | pRS416 | Empty centromer vector URA3 |
| Recombinant DNA reagent | YCp5O-GCN4 (Plasmid) | Hinnebusch, 1985 | p180 | Wild type GCN4 (centromeric plasmid, URA3 selection) |
| Recombinant DNA reagent | YCp5O-GCN4C (Plasmid) | Mueller et al., 1987 | p227 | Constitutively active GCN4 (centromeric plasmid, URA3 selection) |
| Recombinant DNA reagent | pRS416-CAN1-GFP (Plasmid) | Lin et al., 2008 | pCHL571 | GFP-tagged version of CAN1 (centromeric plasmid, URA3 selection) |
| Recombinant DNA reagent | pRS416-MUP1-GFP (Plasmid) | Lin et al., 2008 | pCHL642 | GFP-tagged version of MUP1 (centromeric plasmid, URA3 selection) |
| Recombinant DNA reagent | pRS416-ART2-6xHIS-TEV-3xFLAG (Plasmid) | This paper | pIS001 | HTF-tagged version of ART2 (centromeric plasmid, URA3 selection; for cloning primer seesupplementary file 4) |
| Recombinant DNA reagent | pRS416-ART2 P748,749A,Y750A-6xHIS-TEV-3xFLAG (Plasmid) | This paper | pIS003 | PxY mutant ofART2-HTF (centromeric plasmid, URA3 selection; for cloning primer see Supplementary file 4) |
| Recombinant DNA reagent | pRS416-ART2 K664,667D,R665,666D-6xHIS-TEV-3xFLAG (Plasmid) | This paper | pIS004 | Basic patch mutant of ART2-HTF (centromeric plasmid, URA3 selection; for cloning primer see supplementary file 4) |
| Recombinant DNA reagent | pRS415-ART2-6xHIS-TEV-3xFLAG (Plasmid) | This paper | pJZ001 | HTF-tagged version of ART2 (centromeric plasmid, LEU2 selection; for cloning primer seesupplementary file 4) |
| Recombinant DNA reagent | pRS415-pART2*-ART2-6xHIS-TEV-3xFLAG (Plasmid) | This paper | pJZ002 | Promotor mutant of ART2-HTF (centromeric plasmid, LEU2 selection; for cloning primer seesupplementary file 4) |
| Recombinant DNA reagent | pRS416-pART2-ART1-6xHIS-TEV-3xFLAG (Plasmid) | This paper | pOS258 | ART1-HTF expressed under ART2 promoter (centromeric plasmid, URA3 selection; for cloning primer see supplementary file 4) |
| Recombinant DNA reagent | pRS416-MUP1-pHluorin (Plasmid) | This paper | pLZ78 | pHluorin-tagged version ofMUP1, obtained from the Emr lab (previously unpublished) (centromeric plasmid, URA3 selection) |
| Recombinant DNA reagent | pRS415-MUP1-GFP (Plasmid) | Schmidt et al., 2017 | pMM02 | GFP-tagged version of MUP1 (centromeric plasmid, LEU2 selection) |
| Recombinant DNA reagent | pRS416-FUR4-GFP (Plasmid) | MacGurn et al., 2011 | pSR21 | GFP-tagged version of FUR4 (centromeric plasmid, URA3 selection) |
| Recombinant DNA reagent | pRS415-ART1 (Plasmid) | This paper | pVI009 | untagged version of ART1 (centromeric plasmid, LEU2 selection; for cloning primer see Supplementary file 4) |
| Recombinant DNA reagent | pRS416-pTDH3-ART2-6xHIS-TEV-3xFLAG (Plasmid) | This paper | pVI013 | Overexpression of ART2-HTF (centromeric plasmid, URA3 selection; for cloning primer seeSupplementary file 4) |
| Antibody | α-FLAG M2 (Mouse monoclonal) | Sigma, Austria | F1804 (RRID:AB_259529) | WB (1:10.000) |
| Antibody | α-GFP IgG1K (Mouse monoclonal) | Sigma, Austria | 11814460001 (RRID:AB_390913) | WB (1:1000) |
| Antibody | α-Pgk1 22C5D8 (Mouse monoclonal) | Invitrogen, USA | 459250 (RRID:AB_2532235) | WB (1:10.000) |
| Antibody | α-ubiquitin P4D1 (Mouse monoclonal) | Santa Cruz Biotechnology, USA | 3936S (RRID:AB_628423) | WB (1:500) |
| Antibody | goat a-mouse (polyclonal)-peroxidase | Sigma | A4416 (RRID:AB_258167) | WB (1:5000) |
| Commercial assay or kit | RevertAid First Strand cDNA Synthesis Kit | Thermo Fisher, USA | K1622 | |
| Commercial assay or kit | RNeasy Mini Kit | Qiagen, Germany | 74104 | |
| Commercial assay or kit | TaqMan Assay ECM21/ART2 | Thermo Fisher, USA | Sc04099967_s1 | |
| Commercial assay or kit | TaqMan Assay PGK1 | Thermo Fisher, USA | Sc04104844_s1 | |
| Commercial assay or kit | TaqMan Gene Expression Master Mix | Thermo Fisher, USA | 4369016 | |
| Commercial assay or kit | Non-essential gene deletion strain collection in BY4742 | Open Biosystems, USA | YSC1054 | |
| Chemical compound, drug | GFP-Trap_MA Magnetic Agarose beads | ChromoTek, Germany | gtma-20 | |
| Chemical compound, drug | Nonidet P 40 | Fluka, Germany | 74385 | |
| Chemical compound, drug | Yeast Nitrogen Base without amino acids | Roth, Germany | HP26.1 | |
| Chemical compound, drug | Yeast Nitrogen Base without amino acids and ammonium sulfate | VWR, USA | J630-100G | |
| Software, algorithm | Fiji | Schindelin et al., 2012 | Version 1.0 | |
| Software, algorithm | GuavaSoft | Luminex | Version 2.7 | |
| Software, algorithm | Illustrator CS5.1 | Adobe | Version 15.1.0 (RRID:SCR_010279) | |
| Software, algorithm | ImageJ2 | Rueden et al., 2017 | Version 2.0.0-rc49/1.51 hr (RRID:SCR_003070) | |
| Software, algorithm | Photoshop CS5 | Adobe | Version 12.0.4 × 64 (RRID:SCR_014199) | |
| Software, algorithm | PikoReal | Thermo Fisher | version 2.2 | |
| Software, algorithm | VisiView | Visitron | version 2.1.4 |