Fig. 10.

(a) Optical schematic of a non-orthogonal single-objective (NOSO) LSM system, showing the illumination (blue) and collection (green) light paths. (b) Inset of the shared primary objective of a NOSO system showing the angled light sheet and collection path. (c,d) Inset of the remote focus of a NOSO system (c) without a refractive prism, showing some light is lost at the remote focus and (d) with a refractive prism, showing collection of otherwise lost light. CL: cylindrical lens, TL: tube lens, DBS: dichroic beam splitter.