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. 2020 Aug 1;10(21):9721–9740. doi: 10.7150/thno.44342

Figure 6.

Figure 6

HI-511 binds to and effectively suppresses the activation of AURKB and BRAF V600E. (A) The structure of HI-511. (B) Computer docking model of HI-511 binding with AURKB. (C) Computer docking model of HI-511 binding with BRAF V600E. (D) HI-511 inhibits AURKB in vitro. Active kinase AURKB and HI-511 (0, 1, 5 and10 µM) or APIO-EE-9 (5 µM) were mixed with the substrate histone H3. The relative amounts of phosphorylated substrate were visualized by Western blot. (E) HI-511 inhibits BRAF V600E in vitro. Active kinase BRAF V600E and HI-511 (0, 1, 5, 10 µM) or vemurafenib (5 µM) were mixed with the substrate phosphatidylinositol. The relative amounts of phosphorylated substrate were visualized by Western blot.