Table 1.
List of expression primers used in this study
PCR | Primer | Sequence (5'-3') | Product size (bp) | PCR Cycles | Annealing temperature (°C) |
---|---|---|---|---|---|
RT-PCR | ZDHHC1F | CCTGAGAAGAGTGTGTGGAC | 277bp | 35 | 55 |
ZDHHC1R | CATCTGCTGGATCGATGGAG | ||||
β-actinF | TCCTGTGGCATCCACGAAACT | 315bp | 23 | 55 | |
β-actinR | GAAGCATTTGCGGTGGACGAT | ||||
qRT-PCR | NLRP3F | ACAGCCACCTCACTTCCAG | 168bp | 60 | |
NLRP3R | CCAACCACAATCTCCGAATG | ||||
NFKBF | GGGGCACGATTGTCAAAGA | 119bp | 60 | ||
NFKBR | GGGGACTACGACCTGAATGC | ||||
IL1BF | CTCCAGGGACAGGATATGGA | 194bp | 60 | ||
IL1BR | TTCTGCTTGAGAG GTGCTGA | ||||
IL18F | ACAGCTTCGGGAAGAGGAAAGGAA | 127bp | 60 | ||
IL18R | TGTCTTCTACTGGTTCAGCAGCCA | ||||
CHOPF | AGAGATGGCAGCTGAGTCAT | 177bp | 60 | ||
CHOPR | GCAGGGTCAAGAGTGGTGAA | ||||
GRP78F | AACACGGTCTTTGACGCCAAG | 143bp | 60 | ||
GRP78R | GTTTGCCCACCTCCAATATCAAC | ||||
GRP94F | AGAACCTGCTGCATGTCACAG | 181bp | 60 | ||
GRP94R | GCGGAATAGAAACCGACACCA | ||||
EIF2AF | TACTGCACTCCTTCGACCTC | 114bp | 60 | ||
EIF2AR | GTATCCCAGCTGTGCCATCT | ||||
ATF4F | CGGGACAGATTGGATGTTGGAG | 182bp | 60 | ||
ATF4R | TTGGGTTCACCGTCTGGGG | ||||
PERKF | TATGGACTCAGTGCATATAGTGG | 162bp | 60 | ||
PERKR | CTTCTCATTGCCACTGCGAG | ||||
ATF6F | CACAGACACTGATGAGCTGCA | 129bp | 60 | ||
ATF6R | CAGATTTGGTTGTTGATGTCCCA | ||||
XBP1F | CCTGGTTGCTGAAGAGGAGG | 136bp | 60 | ||
XBP1R | CCATGGGGAGATGTTCTGGAG | ||||
IRE1F | GCAGGACATCTGGTATGTTAT | 137bp | 60 | ||
IRE1R | GCTACATGGTGATGGTGTATTC | ||||
TNFaF | GCAGTCAGATCATCTTCTCG | 140bp | 60 | ||
TNFaR | TTATCTCTCAGCTCCACGCC | ||||
Caspase1F | TACAGACAAGGGTGCTGAAC | 234bp | 60 | ||
Caspase1R | GTACTCCTTGAGAGTCTTGC | ||||
β-actinF | GTCTTCCCCTCCATCGTG | 113bp | 60 | ||
β-actinR | AGGGTGAGGATGCCTCTCTT |
Note. RT-PCR: Semiquantitative real-time PCR, qPCR: quantitative real-time PCR.