Table 2.

List of MSP primers used in this study

MSP	Primer	Sequence (5'-3')	Product size (bp)	PCR Cycles	Annealing temperature (°C)
MSP	ZDHHC1m1	ATTTATCGGTTTAGTTTTCGTTC	112bp	40	60
	ZDHHC1m2	CTCCCCGAAACAACCGCG
	ZDHHC1u1	GATTTATTGGTTTAGTTTTTGTTT	114bp	40	58
	ZDHHC1u2	CCTCCCCAAAACAACCACA
BGS	ZDHHC1BGSF	TTAAGAGGGTTTTTGGTAAGTT	396bp	40	60
	ZDHHC1BGSR	TAAACCCTACCAAAATCAACC

Note. MSP: methylation-specific PCR, BGS: Bisulfite genomic sequencing.