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. 2020 Aug 6;10(21):9899–9912. doi: 10.7150/thno.45133

Figure 5.

Figure 5

Effects of Arrb2 on angiogenic function. (A-C) EPCs were infected with Lv-Arrb2 or Lv-GFP for 48 h. Representative images and quantification of tube formation (A) showing the effects of Lv-Arrb2 on angiogenic function in vitro. Scale bar = 400 µm. Representative images and quantification of immunohistochemistry (B) and anti-CD31 staining (C) of sections of Matrigel plugs were presented to show the effects of Lv-Arrb2 on EPCs angiogenesis ex vivo. Scale bar = 200 µm. (D-F) EPCs were infected with sh-Arrb2 or sh-Con for 48 h. Representative images of tube formation (D) showing the effects of sh-Arrb2 on angiogenic function in vitro. Scale bar = 400 µm. Representative images and quantification of immunohistochemistry (E) and anti-CD31 (F) staining of sections of Matrigel plugs showing the effects of sh-Arrb2 on EPCs angiogenesis ex vivo. Scale bar = 200 µm. All data were expressed as mean ± SD (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group.