Figure 6.

Effects of Arrb2 on ERK and Akt activation in EPCs. (A and B) EPCs were infected with Lv-Arrb2 or Lv-GFP for 48 h. The effects of Lv-Arrb2 on ERK1/2 and Akt phosphorylation were determined by Western blot analysis (A) and then quantitated by densitometric analysis (B). (C and D) EPCs were infected with sh-Arrb2 or sh-Con for 48 h. The effects of sh-Arrb2 on ERK1/2 and Akt phosphorylation were determined by Western blot analysis (C) and then quantitated by densitometric analysis (D). (E) Protein expression of ERK1/2 and Akt precipitated by Arrb2 from EPCs was examined by Western blot analysis. (F) Protein expression of Akt and PP2A precipitated by Akt from EPCs under normoxic and hypoxic conditions was examined by Western blot analysis. All data were expressed as mean ± SD (n = 3). ^*P < 0.05, ^**P < 0.01 compared with the control group.