Figure 5.

Chemoresistance in GCSCs. Cell viability was quantified using MTS assay after 72 h of bleomycin (BLM), paclitaxel (PXL) and cisplatin (CDDP) treatment in increasing concentrations in MKN45 and SNU638 cell lines cultured in adherence (ADH) or as sphere (CSCs), after MAD2 downregulation (CSCs shM) or not (CSCs WT). Results are presented as the percentage of viable cells relative to untreated cells. Data represent the mean values obtained in three experiments performed in quadruplicate. The statistical significance was evaluated with one-way ANOVA, followed by post hoc comparisons (Bonferroni´s test, *P < 0.05; **P < 0.01; ***P < 0.001).