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. 2020 Aug 1;10(21):9702–9720. doi: 10.7150/thno.44297

Figure 1.

Figure 1

Fgl2 expression was enhanced concomitantly with hepatic macrophage accumulation in patients with NASH. Liver sections of patients with NAFL and NASH and controls (human subjects without NAFLD) were stained with HE (A). CD68+ macrophages (red) and fgl2 (green) were observed by immunofluorescent staining (C). The NAFLD active score was evaluated in controls (n=6), NAFL (n=6) and NASH (n=8) human subjects (B). The fluorescence intensity of fgl2-, CD68- and fgl2-positive macrophages (fgl2 in CD68+) was evaluated by ImageJ software (C). Correlations between fgl2 expression in liver/hepatic macrophages and NAFLD activity score were also analyzed (D). Five microscopic fields per liver section from 3 patients in each group were counted. The data represent the mean ± SD from at least three independent experiments. Statistical differences were determined by one-way ANOVA with Bonferroni correction and Spearman's rank correlation coefficient analysis was applied to analyze the correlation between fgl2 expression levels and NAFLD activity score. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant.