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. 2020 Aug 2;10(21):9830–9842. doi: 10.7150/thno.45161

Figure 1.

Figure 1

The expression and function of SIX1 in hepatocellular cancer. A The level of SIX1 was analyzed in paired HCC specimens and their peritumoral tissues by western blot. B Quantitative analysis of SIX1 expression in paired HCC tumor tissues and normal tissues. C Overall survival (OS) curve of HCC patients in correlation with the expression of SIX1 using Kaplan-Meier Plotter database (n=364). D Western blot and quantitative analysis of SIX1 expression using a panel of HCC cell lines and the immortal liver cell line THLE3. E and F Analysis of cell growth using CCK8 (E) and colony formation (F) assays upon manipulation of SIX1. BEL7402 and BEL7404 cells were transduced with a SIX1 overexpression or knockdown lentivirus, respectively. G Tumors weights of nude mice were measured after four weeks at the experimental endpoint. H Plot of tumor volumes in nude mice measured every week (The data from B, F (left panel), G were analyzed by Student's t-test, data from D and F (right panel) were analyzed by one-way ANOVA and data from E and H were analyzed by two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001).