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. 2020 Aug 2;10(21):9830–9842. doi: 10.7150/thno.45161

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O-GlcNAcylation has reverse effect on SIX1 expression in HCC. A Relationship between O-GlcNAcylation and SIX1 expression levels in fifty HCC samples. BEL7402 and BEL7404 were treated with OGA inhibitors or transduced with OGT lentivirus (overexpression or knockdown) as indicated. B SIX1 expression levels were analyzed by western blotting in established HCC cell lines with different manipulation of O-GlcNAcylation. C SIX1 expression in BEL7402 cells treated with an OGA inhibitor (TMG) at the indicated times. D The direct target genes of SIX1 were analyzed in established HCC cell lines. E Immunoprecipitation with anti-OGT antibody followed by western blotting with anti-SIX1 antibody. Same experiment was performed when SIX1 was immunoprecipitated and immunoblotted with anti-OGT antibody. F O-GlcNAcylation modified protein immunoprecipitated from cell extracts was analyzed by immunoblotting for anti-SIX1.