Figure 5.

O-GlcNAcylation stabilizes SIX1 via CDH1. A The mRNA levels of SIX1 were analyzed in HCC cell lines as indicated. B Levels of SIX1 were determined by western blotting in BEL7402 cells treated with CHX (10 μg/ml) for the indicated times. C Ubiquitination of SIX1 was examined in BEL7402 cells after OGA inhibitor treatment. MG132 and NEM were used to inhibit proteasome and deubiquitination, respectively. D CDH1 was detected in purified SIX1 immunoprecipitation samples from BEL7402 treated with DMSO or TMG. E SIX1 expression was examined in BEL7402 CDH1-overexpressing cells with an increasing amount of TMG. (The data from right panel of A and E were analyzed by one-way ANOVA, data from B were analyzed by two-way ANOVA, and the rest of the statistics were analyzed by Student's t-test. ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001).