Figure 7.

Depletion of OGT reverses the tumor-promoting effect of SIX1 overexpression. A SIX1 downstream factor protein levels and quantitative analysis data in BEL7402 treated as indicated were examined by western blot. B and C CCK8 (B) and Colony formation (C) assays were performed in BEL7402 cells infected with the indicated lentivirus. D Tumors weights of nude mice were measured after four weeks at the experimental endpoint. E Plot of tumor volumes in nude mice was measured every week. F Schematic model of O-GlcNAcylation and SIX1 in HCC progression. The sign “G” indicates the O-GlcNAc and “Ubi” indicates the ubiquitin. (The data from A, C and D were analyzed by one-way ANOVA, and data from B, E were analyzed by two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001).