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. 2020 Aug 26;10:14213. doi: 10.1038/s41598-020-70855-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-immunoprecipitation analysis for the interaction of Udu with Bap1, Dnmt1, Thoc1, and Cry3a. Flag-tagged control or Flag-tagged Udu-expressing constructs were co-transfected with different Myc-tagged candidate Udu-interacting proteins, including Bap1, Dnmt1, Thoc1, and Cry3a. The cell lysate was incubated with anti-FLAG M2 magnetic beads, then subjected to immunoblot with anti-MYC or anti-FLAG antibody. Arrowheads indicate the expected size of full-length Udu. Lanes 1 to 9 were cropped from the same blots, while lanes 10–12 were from the other ones. To observe the expression of full-length Udu, photographs using a longer exposure time of anti-FLAG immunoblots were taken. The original un-cropped immunoblots were shown in Supplementary Fig. S7a and photographs with shorter exposure time for anti-FLAG immunoblots were also included in Supplementary Fig. S7b as supporting information.