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. 2020 Aug 26;10:14213. doi: 10.1038/s41598-020-70855-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Whole mount in situ hybridization analysis for temporal and spatial expression of the bap1, dnmt1, thoc1, cry3a, and udu genes during zebrafish early development. WISH of (a–d’) bap1, (e–h’) dnmt1, (i–l’) thoc1, (m–p’) cry3a, and (q–t’) udu expression at 24 hpf (a,b,e,f,i,j,m,n,q,r) and 72 hpf (c,d’,g,h’,k,l’,o,p’,s,t’) embryos. Black arrowhead indicates the pronephric duct (q). Rhombomere boundaries are indicated by black lines (r). Black asterisks indicate otic vesicles (f,j,n,r). Red arrows indicate olfactory sensory epithelium (c,k). Dashed rectangles in (d,h,l,p,t) are shown as enlarged images in (d’,h’,l’,p’,t’), respectively. Black arrows indicate neuromasts of the posterior lateral line (d’,h’,l’,p’,t’) and white dash lines indicate notochord (t’). Flat mount of WISH samples: (b,f,j,n,r). All embryos are oriented with anterior to the left.