Table 1.
Zebrafish Udu-binding proteins isolated by yeast two-hybrid analysis.
| Category | Bait | PBS* | Prey binding region | Symbol | Name (accession number) | GO terms |
|---|---|---|---|---|---|---|
| Lipid metabolism-related | 1 | D | unknown-3786 | Apobb.1 | Apolipoprotein Bb, tandem duplicate 1 (apobb.1), mRNA (NM_001030062.1 ) | Lipid transporter activity |
| 1 | D | − 1 ~ 399 | Ephx2 | Epoxide hydrolase 2, cytoplasmic, transcript variant X1, mRNA (XM_021467153.1) | Epoxide hydrolase activity | |
| Transcriptional regulation | 1 | D | 432 ~ 1,248 | Pax7b | Paired box 7b (NM_001146149.1) | Sequence-specific DNA binding |
| 2 | D | 92 ~ 652 | Hoxc4a | Homeobox C4a, mRNA (NM_131122.2) | Sequence-specific DNA binding | |
| 1 | D | 96 ~ 600 | Otx1 | Orthodenticle homeobox 1, mRNA (NM_131250.2) | Sequence-specific DNA binding | |
| 1 | D | − 1 ~ 954 | Cry3a | Cryptochrome circadian regulator 3a, transcript variant 1, mRNA (NM_001313822.1) | Negative regulation of transcription; sequence-specific DNA binding | |
| 2 | D | − 7 ~ 754 | Pelp1 | Proline, glutamate and leucine rich protein 1, mRNA (XM_001338982.7) | Transcription factor binding; chromatin binding | |
| RNA-binding/processing | 1 | D | 2094 ~ 2,781 | Heatr6 | HEAT repeat containing 6 (NM_001114313.1) | RNA binding |
| 2 | D | − 1 ~ 614 | Fbrsl1 | Fibrosin-like 1 protein variant 1a mRNA, complete cds, alternatively spliced (KY492379.1) | RNA binding | |
| 2 | D | − 1 ~ 1,026 | Rbmx2 | RNA binding motif protein X-linked 2, mRNA (NM_001025166.1) | RNA binding | |
| Apoptosis/Cell proliferation/DNA replication | 2 | D | 1,155 ~ 1893 | Mcm3 | Minichromosome maintenance complex component 3, mRNA (NM_212567.2) | DNA replication origin binding; helicase activity |
| 2 | D | 1971 ~ 2,229 | Mcm4 | minichromosome maintenance complex component 4, mRNA (NM_198913.2) | DNA replication origin binding; helicase activity | |
| 1 | D | − 1 ~ 1,355 | Bnipl | BCL2/adenovirus E1B 19kD interacting protein, like, transcript variant X1, mRNA (XM_009292526.3) | Exopolyphosphatase activity | |
| 2 | D | 270 ~ 1744 | Gulp1b | GULP, engulfment adaptor PTB domain containing 1b, transcript variant X5, mRNA (XM_001345116.6) | Apoptotic process | |
| 2 | D | 135 ~ 706 | Gpatch11 | G patch domain containing 11, mRNA; zgc92714 (NM_001002393.1) | Nucleic acid binding | |
| Signaling pathways | 1 | D | 1,356 ~ unknown | Dlc | deltaC, mRNA (NM_130944.1) | PDZ domain binding; Notch binding |
| 1 | D | Unknown ~ 1,216 | Napba | N-ethylmaleimide-sensitive factor attachment protein, beta, mRNA (NM_001020655.2) | Soluble NSF attachment protein activity; syntaxin binding | |
| 2 | D | 2,640 ~ 3,309 | Dock6 | Danio rerio dedicator of cytokinesis 6, transcript variant X1, misc_RNA (XR_660514.3) | Guanyl-nucleotide exchange factor activity | |
| Protein translocation/ processing | 1 | D | Unknown ~ 915 | Ssr1 | Signal sequence receptor, alpha, mRNA (NM_201327.1) | Cellular response to estrogen |
| 1 | D | − 1 ~ 559 | Naa15b | N(alpha)-acetyltransferase 15, NatA auxiliary subunit b, mRNA (NM_203321.2) | Peptide alpha-N-acetyltransferase activity | |
| Cytoskeleton | 1 | D | − 1 ~ 628 | Agrn | Agrin, mRNA (NM_001177452.1) | Serine-type endopeptidase inhibitor activity |
| 1 | D | − 1 ~ 608 | Pleca | plectin a, mRNA (NM_001100032.1) | Ankyrin binding; actin binding | |
| 1 | D | 147 ~ 841 | Actc1 | cardiac muscle alpha actin 1 (NM_001002066.1) | Nucleotide binding; ATP binding | |
| 2 | D | − 1 ~ unknown | Neb | Nebulin (neb), transcript variant X41, mRNA (XM_021474182.1) | Actin filament binding | |
| 2 | D | − 1 ~ unknown | Cnn3b | Calponin 3, acidic b, mRNA (NM_001024073.1) | Actin binding; calmodulin binding | |
| Others | 1 | D | 225 ~ 661 | Slc25a3b | Solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3b, mRNA (NM_213722.1) | Inorganic phosphate transmembrane transporter activity |
*PBS is a score that is automatically computed through algorithms; D means “moderate confidence in the interaction”.