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. 2020 Aug 26;11(8):705. doi: 10.1038/s41419-020-02872-5

Fig. 4. ASF1B knockdown arrested the cell cycle.

Fig. 4

a Flow cytometry was used to detect the cell cycle changes in HeLa cells with ASF1B silencing. More stable ASF1B-shRNA cells remained in the G2/M stage than scrambled cells. b Accumulated analysis of the cell cycle. c Western blots were performed to detect the cell cycle-related proteins cyclin A1, cyclin B1, and cyclin D1. d Flow cytometry was used to detect cell cycle changes in HeLa cells overexpressing ASF1B. No significant cell cycle alterations were found when comparing ASF1B-overexpressing cells and vector cells. e Accumulated analysis of the cell cycle. f Western blots were performed to detect the cell cycle-related proteins cyclin A1, cyclin B1, and cyclin D1.