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. 2020 Aug 26;10:14210. doi: 10.1038/s41598-020-71041-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Calibration curve between the number of L3.6pl cells captured in the LFAM2 device and the number of cells spiked in healthy blood. (B) CTCs per mL of blood samples from cancer patients, enumerated using either LFAM2 or GEM. (C) Box plots of the results in (B) with the average and range of CTCs per mL. (D) Comparison between two blood preparation methods, Ficoll-Paque and 2-time diluted whole blood, prior to infusion into LFAM2.