Fig. 1. ADSC treatment alleviates neovascularization associated with corneal wound healing.
a–f The eyes of male BALB/c mice were treated with or without (control group) 75% ethanol (EtOH & ADSCs group). The ADSCs group mice were injected with human ADSCs (5 × 105 cells per mouse) 1 h after injury; control and EtOH groups treated with PBS were used as negative and positive controls. The bright-field photo of eyeballs (a) and their inverted phases (b) were taken in each group on Day 21. Vascular areas of corneas in each group (c) were presented as pixels (n = 4–5 mice/group). d The CD31 expression of the whole eyeball in each group was detect by Western blotting analysis, each band represents a different eyeball sample. The level of GAPDH in each sample was used as a loading control. e Immunofluorescence images of the whole cornea and the antibodies against CD31 was used to detect blood vessels. Scale bars represent 50 μm. Fluorescence photographs (f) of corneas in different groups were taken on Day 21, and sodium fluorescein (2.5 μg/mice) was injected into the tail vein before the methanation of the mice. Results are means ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001 determined by one-way ANOVA with Tukey comparisons.