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. 2020 Aug 26;11(8):707. doi: 10.1038/s41419-020-02914-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a–g Mice were treated as described in Fig. 1. a The absolute numbers of total and CXCR4^high neutrophils in eyeballs were detected by flow cytometry on Day 3 (n = 10–11 mice/group). b, c Mice with ethanol injured eye were treated with/without AMD3100 and ADSCs, the absolute numbers of total and CXCR4^high neutrophils (left b and c) in eyeballs were detected by flow cytometry on Day 7. And the vascular areas (right b) were also measured on Day 7 after AMD3100 treatment (n = 8 mice/group in B, n = 14–15 mice/group in (c). d The absolute numbers of total and CXCR4^high neutrophils in lungs were detected by flow cytometry on Day 7 (n = 8 mice/group). e The mRNA levels of Cxcl-12 in the eyeball were determined by qPCR normalized to β-actin (n = 10 mice/group) on Day 3. f, g The protein levels of Cxcl-12 in the lung explant supernatant and serum were determined by ELISA (n = 12 mice/group) on Day 3. Data are shown as means ± SEM, *P < 0.05; ***P < 0.001; ****P < 0.0001 determined by one-way ANOVA with Tukey comparisons.