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. 2020 Aug 26;10:14205. doi: 10.1038/s41598-020-69907-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ADSC induces the activation of the NF-kappa B pathway in SiHa and CaSki cells. (a–h) The photographs show the expression of RelB and p52 obtained by immunofluorescence of SiHa (a–c) or CaSki (e–g) cells cultured with serum-free DMEM or ADSC-CM (free of serum) for 24 h. The images were taken under a confocal microscope. The scale bar = 40 μm. The cell nuclei were contrasted with DAPI. Each experiment was repeated at least three times. To quantify the expression levels, the intensity of fluorescence was quantified using Image J. Graphs show the mean fluorescence intensity of RelB (b), p52 (d) in SiHa and Relb (f) and p52 (h) in Caski cells. Graph represents three biological replicates, error bars are s.d. and ****p < 0.0001 and *** p < 0.001.