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. 2020 Aug 26;10:14195. doi: 10.1038/s41598-020-71134-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of E6 to AIF. Expression plasmids (shown at the top) were co-transfected into 293TT cells by using PEI. Then, proteins in the cell lysates were pulled down using Streptavidin Mag Sepharose and immunoblotted with anti-HA-tag and anti-SBP antibodies. (a) Confirmation of HPV16 E6 binding to AIF. (b) Confirmation of the binding of E6 from HPV16 and HPV6 to three AIF forms. Numbers on the left are the molecular masses (in kDa). Five independent experiments were performed and one of them is shown. PD: pulldown; IB: immunoblot.