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. 2020 Aug 26;10:14195. doi: 10.1038/s41598-020-71134-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proteasomal degradation of AIF induced by E6. 293TT cells co-transfected with pAIF-HA and p16E6 (a) or p6E6 (b) by using PEI. After 24 h, the cell lysate was immunoblotted with anti-HA-tag and anti-SBP antibodies. pGFP-SBP was used as a transfection internal control. In some samples, cells were treated with MG132 12 h prior to cell harvest. Five independent experiments were performed and one of them is shown. Numbers on the left are molecular masses (in kDa). IB: immunoblot.