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. 2020 Aug 26;10:14195. doi: 10.1038/s41598-020-71134-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of AIF-induced chromatin degradation by HPV16 E6. 293TT cells were transfected with p16E6, p6E6, and/or pΔ102AIF expression plasmids. Then, cell lysates from the transfected cells were incubated with nuclei isolated from HeLa cells and stained with propidium iodide (PI). Chromatin degradation was assessed by flow cytometry and quantified by sub-G1 population gating. (a) Representative flow cytometric panels are shown. (b) The percentages of sub-G1 PI-positive cells from three independent experiments performed with five samples were analyzed, *** indicates a significant difference between the two groups. ns, no significant difference. Statistical analyses were performed by Krushal-Wallis test with Steel–Dwass test.