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. 2020 Aug 26;10:14195. doi: 10.1038/s41598-020-71134-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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E6 from HPV16 inhibits AIF-mediated apoptosis in MEF cells. (a) Normal MEF cells, HPV16 E6-expressing (MEF-16E6) and HPV6 E6-expressing (MEF-6E6) MEF cells were treated with or without STS (a protein kinase inhibitor). Four hours after STS stimulation, cells were stained with PI and FITC-Annexin V and analyzed by flow cytometry. Annexin V + PI- and Annexin V + PI + cells were defined as early and late apoptotic cells, respectively. (b) Normal MEF, MEF-16E6, and MEF-6E6 cells were transfected with siControl or siAIF twice with a 6 h interval and then treated with STS for 4 h. Cells were stained with PI and FITC-Annexin V and analyzed by flow cytometry. Normal MEF cells were included as a negative control. Raw data were shown in (b). The relative effect of siAIF compared with siControl was shown in (c). Each sample was analyzed in quadruplicate. Three independent experiments were performed and one of them is shown. *** indicates a significant difference between the groups (p < 0.05). Statistical analyses were performed by the Mann–Whitney U test for two group comparison and by Krushal-Wallis test with Steel–Dwass test for multi-group comparison.