Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 26;11:4263. doi: 10.1038/s41467-020-18067-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a SDS-PAGE gel of purified Drosophila loading factors, either full-length proteins or constructs lacking IDRs (ΔN). b DmMcm2–7 is recruited (low-salt wash) and loaded (high-salt wash) in an ATP-dependent manner by full-length and IDR-less DmOrc1, DmCdc6, and DmCdt1 with similar efficiency. Note that FL-Cdt1, Cdt1ΔN, Cdc6, Cdc6ΔN, and to some extent FL-Orc1 associate with beads nonspecifically. c Loaded DmMcm2–7 are double-hexamers. DmMcm2–7 before and after loading was analyzed by negative-stain EM. 2D class averages show Mcm2–7 single-hexamers before loading but double-hexamers after loading. Source data are provided as a Source data file.