. 2020 Jun 22;295(33):11693–11706. doi: 10.1074/jbc.RA120.013678

Table 1.

Parameters describing the equilibrium binding of eIF4GI constructs and DAP5 to the 5′ UTRs^a

5′ UTR	eIF4GI_557-1599			eIF4GI_682-1599			DAP5
5′ UTR	K_d ± S.D. (nm)	Amp. (r_max–r_min)	χ²	K_d ± S.D. (nm)	Amp. (r_max–r_min)	χ²	K_d ± S.D. (nm)	Amp. (r_max–r_min)	χ²
HIF1α	50 ± 6.0	0.045	0.996	139 ± 23.0	0.034	0.994	154 ± 18.0	0.061	0.988
FGF-9	12 ± 2.0	0.079	0.995	120 ± 7.0	0.027	0.996	136 ± 10.0	0.039	0.998
p53_A	86 ± 4.0	0.021	0.998	248 ± 16.0	0.036	0.999	290 ± 14.0	0.044	0.999
p53_B	68 ± 19.0	0.025	0.998	126 ± 4.0	0.017	0.998	112 ± 6.0	0.048	0.998

^a K_d is the equilibrium dissociation constant, r_max–r_min is the amplitude (Amp.) that indicates change in anisotropy, and χ² represents the goodness of fit.