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. 2020 Jun 22;295(33):11693–11706. doi: 10.1074/jbc.RA120.013678

Table 2.

Primers used

Reporter Forward primer Reverse primer
Used for the cloning of the UTRs in reporter constructs
    HIF1α 5′-CTAGGCGGCCGCTAATACGAC-3′ 5′-CTAGGCGCGCGGTGAATCGGTC-3′
    FGF9 5′-CTAGGCGGCCGCTAATACGAC-3′ 5′-CTAGGCGCGCCAGAGGACTCGGC-3′
    p53A 5′-CTAGGCGGCCGCTAATACGAC-3′ 5′-CTAGGCGCGCGGCAGTGAC-3′
    p53B 5′-CTAGGCGGCCGCTAATACGAC-3′ 5′-CTAGGCGCGCTGCTTGGGAC-3′
    β-actin 5′-CTAGGCGGCCGCTAATACGAC-3′ 5′-CTAGGCGCGCGGTGAGCTGG-3′
Used for insertion of stable hairpin at 5′UTR end of UTR-Luc constructs
    HIF1α 5′-AGGCCGTGCACAGTGCTGCCTCG-3′ 5′-AGGCCAAGCCTATAGTGAGTCGTATTAG-3′
    FGF9 5′-AGGCCGAAACAGCAGATTACTTTTATTTATG-3′ 5′-AGGCCTATAGTGAGTCGTATTAGC-3′
    p53A 5′-AGGCCGGTCTAGAGCCACCGTCC-3′ 5′-AGGCCCTATAGTGAGTCGTATTAGCGG-3′
    p53B 5′-AGGCCATGGAGGAGCCGCAGTCA-3′ 5′-AGGCCCCCTATAGTGAGTCGTATTAGCG-3′
    β-actin 5′-AGGCCACCGCCGAGACCGCGTCC-3′ 5′-AGGCCCCTATAGTGAGTCGTATTAGCGCGCG-3′