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GEMMA spectra recorded on HydF and HydA1 proteins with different cofactor loadings. A, HydA1 (top, holo-HydA1; middle, [4Fe4S]-HydA1; bottom, apo-HydA1); B, HydF (top, holo-HydF; middle, [4Fe4S]-HydF; bottom, apo-HydF). The protein concentration in all samples were 0.02 µg/µl in 20 mm ammonium acetate and 0.005% Tween-20.
