Figure 1.

Stability and unfolding kinetics of RNH I53D measured by CD at pH 7.4. A, equilibrium urea-induced denaturant melt (black circles) of RNH I53D (pH 7.4 at 25 °C). The data were fit using a two-state approximation (U $\leftrightharpoons \hspace{0.17em}$ N, where U and N are the unfolded and native states, respectively), and the data shown are from one representative experiment of three. The average C_m from three experiments is marked by a red square with an error bar for the S.D. B, natural log of the observed unfolding rate for RNH I53D at pH 7.4 as a function of urea.