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Stability of RNH D10A/I53D. The off-ribosome values are from three experiments, and the on-ribosome values are from two experiments
| RNH D10A/I53D (CD)a | RNH D10A/I53D-(GS)5-SecM |
||
|---|---|---|---|
| Off | On | ||
| Cm (m, urea) | 4.25 ± 0.2 | 3.83 ± 0.02 | 3.35 ± 0.14 |
| ΔGunf (kcal/mol) | 8.5 ± 0.4 | 7.66 ± 0.03 | 6.71 ± 0.28 |
| munf (kcal mol−1 m−1) | 2.0 ± 0.1 | ||
a The values are from Connell et al. (32); pH 5.5 buffer without divalent cations.
