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. 2020 Jun 21;295(33):11584–11601. doi: 10.1074/jbc.RA120.012444

Table 1.

Kinetic data for GePTS1, PsPTS1, and GePTS1 mutants^a

	cis-DMI ((3R,4R) and (3S,4S))				trans-DMI ((3S,4R) and (3R,4S))
	K_m	V_max	k_cat	k_cat/K_m	K_m	V_max	k_cat	k_cat/K_m
	μm	picokatals/μg protein	s^–1	m^–1 s^–1	μm	picokatals/μg protein	s^–1	m^–1 s^–1
GePTS1	145	2,674	59.9	412,800	680	712	15.9	23,440
PsPTS1	890	2,285	50.5	56,750	830	147	3.3	3,930
D50A	1,175	220	4.9	4,180	520	9	0.2	370
D83A	1,300	283	6.3	4,870	665	58	1.3	1,960
Y103F	555	306	6.9	12,340	3,320	265	5.9	1,830
Y181F	825	233	5.2	6,310	5,665	180	4.0	710

^a under the assay conditions, cis-DMI (3R,4R) and trans-DMI (3S,4R) were only converted into products.