Figure 2.

Glp1r promoter activity is enriched in β-and δ-cells. A, gating strategy to separate tdTomato+ (Tom+) and GFP+ islet cells from Glp1r:mTmG mice by FACS. B, quantification of Tom+ (red circles), GFP+ (green circles), and GFP+/Tom+ (yellow circles) cells from individual mice. C–F, qPCR in Tom+ and GFP+ cells for expression of (C) Glp1r, (D) Gcg, (E) Ins2, and (F) Sst. All qPCR data are normalized to gene expression in whole islet lysates. Comparisons between Tom+ and GFP+ cells were compared by paired t test, ****, p < 0.0001. Each circle represents an individual mouse and data are presented as mean ± S.E.