Figure 5.

qPCR of FACS-sorted populations from Glp1r:mTmG mice incubated with GLP1R-APC. A–C, sorted populations from Tom⁺/GLP1R-APC⁻ (red bar/gray circles), GFP⁺/GLP1R-APC⁻ (green bar/gray circles), and GFP⁺/GLP1R-APC⁺ (green bar/orange circles) were analyzed for expression of (A) Gcg, (B) Ins2, and (C) Sst. Tom⁺/GLP1R-APC⁺ cells were not acquired. All qPCR data are normalized to gene expression in whole islet lysates. Populations were compared by mixed effects analysis and Tukey's multiple comparisons test was used for pairwise comparisons. Significant differences are denoted by ***, p < 0.001; ****, p < 0.0001. Each circle represents an individual mouse and data are presented as mean ± S.E.