Figure 2.

BCL11A enhances metastatic outgrowth of TNBC cells. A, stable overexpression of BCL11A mRNA (compared with empty vector [EV]) in SUM149PT-FFluc cells was measured by qPCR. B, cell growth was assessed over 6 days using the Cyto-X colorimetric cell counting reagent. C, quantitation of bioluminescence 31 days after intracardiac injection of stable cell lines (EV = 8 mice/group and BCL11A = 9 mice/group). D, number of mice with macrometastases. Significance of differences was calculated in panel A using an unpaired t test, in panel C using a Mann-Whitney test, and in panel D using chi-square test. *, p < 0.05; **, p < 0.01.