EPR spectra of •NO addition to ADO in the absence (A) and presence (B and C) of cysteamine.
•NO was released by 0 (black), 0.1 (gray), 0.5 (light gray), and 2.5 (red) equiv of •NO releasing agent (1 equiv of agent produces approximately 1.5 equiv of •NO). A, in the absence of substrate, changes occur in the low-field (high-spin) region with increasing •NO. Spectra were taken at 10 K with 50 mW microwave power. B, in the presence of substrate, changes occur in both high- and low-spin regions with increasing •NO. Spectra were taken at 10 K, 1 mW. C, in the presence of substrate, changes occur in the g = 2 region with increasing •NO. Spectra were taken at 50 K with 0.05 mW microwave power.