Figure 3.

Sensitivity of TAZ-KO cells to oxidants is rescued by DFO. The cells were seeded equally in 96-well plates and incubated for 3 h until they attached to the plate. 20 μm H₂O₂ (A) or 50 mm FeCl₃ (B) was added, and the cells were incubated for the indicated times, after which cell viability was measured by MTT assay, as described under “Experimental Procedures.” C, TAZ-KO cells were either mock-treated (CTRL) or exposed to 200 μm DFO for 3 h prior to the addition of 20 μm H₂O₂. The data shown are means ± S.D. (n = 3). *, p < 0.05.