Figure 5.

Decreased Yfh1 in yeast crd1Δ cells. A, Western blotting analysis of Nfs1, Yfh1, and Isu1 proteins from mitochondrial extracts of WT, crd1Δ, and yfh1Δ cells grown in SD galactose at 36 °C. Por1 was used as a loading control. The signal intensities of protein bands and surrounding background were scanned and quantified using ImageJ. The background-subtracted value for each protein band was normalized to that of Por1 and quantified relative to WT. The data shown are means ± S.D. (n = 3). *, p < 0.05. B, mRNA levels of YFH1 were quantified by qPCR from WT and crd1Δ yeast cells grown in SD galactose at 30 and 36 °C. The data shown are means ± S.D. (n = 3).