Figure 10.

Glycerol utilization of cells defective in the Nem1-Spo7/Pah1 phosphatase cascade and of spo7Δ cells expressing Spo7 with LLI mutations. A, WT, pah1Δ, nem1Δ, and spo7Δ mutant cells were grown at 30 °C in YPD to saturation, serially diluted (10-fold), and spotted onto agar plates containing 2% glucose or 3% glycerol. B, spo7Δ (GHY67) transformants expressing the indicated SPO7 allele (pGH443 and its derivatives) were grown in SC-Leu medium and treated as in panel A. Growth on glucose and glycerol was scored after 3 and 6 days, respectively, of incubation at 30 °C. The data are representative of three independent experiments.