Figure 8.

Nuclear/ER morphology of cells defective in the Nem1-Spo7/Pah1 phosphatase cascade and of spo7Δ cells expressing Spo7 with LLI mutations. A, WT, pah1Δ, nem1Δ, and spo7Δ cells harboring YCplac111-SEC63-GFP were grown at 30 °C in SC-Leu medium to logarithmic phase, and the fluorescence signal of the GFP-tagged ER marker Sec63 was visualized by fluorescence microscopy. B, spo7Δ (GHY68) transformants harboring pRS413-SEC63-GFP (pGH449) and the indicated SPO7 allele (pGH443 and its derivatives) were grown and examined as in panel A, except that SC-His-Leu medium was used for plasmid selection. The percentage of cells with aberrant nuclear/ER morphology (misshaped versus round nuclei) was determined from ≥4 fields of view (≥200 cells). A, upper, the images shown are representative of multiple fields of view. DIC, differential interference contrast. White bar, 2 μm. A, lower, and B, the data shown are averages from three experiments ± S.D. (error bars). The individual data points are also shown. *, p < 0.05 versus cells expressing the WT control.