Figure 5.
Inhibiting the mTORC1 pathway partially rescues the liver maturation defects in rbm15cq96. A, confocal images (3D) showing the antibody staining of p-4Ebp1 and 2F11 to evaluate the activity of mTORC1 signal in hepatocytes at 5 dpf. B, rapamycin treatment and confocal images reflecting the rescue efficiency of rapamycin treatment. C, quantification of Dendra2 fluorescent intensity in WT, cq96, and rapamycin treatment. D, antibody staining results of Hnf4a and Dendra2 showing that rapamycin treatment affects the cellular and protein levels of Dnedra2 and Hnf4a. E, the quantification of Hnf4a fluorescent intensity in WT, cq96, and rapamycin treatment. F, WISH images showing the expression patterns of cp and gc after rapamycin treatment. rapa, rapamycin. The numbers indicate the proportion of larvae exhibiting the expression shown. Asterisks indicate statistical significance: ****, p < 0.0001. Scale bars, 100 μm; error bars, S.D.